Creatine has become increasingly commonly used by body builders to improve their ability to build muscle tissue. By allowing body builders to perform more work as a result of additional energy, increased protein synthesis is stimulated. Also, when an abundance of creatine phosphate is stored in the muscle, the muscle will hold more water in its cells. The more water is held in a muscle cell, the more it will promote the synthesis of protein as well as deter protein breakdown.
Endogenous serum or plasma creatine concentrations in healthy adults are normally in a range of 2–12 mg/L. A single 5 g (5000 mg) oral dose in healthy adults results in a peak plasma creatine level of approximately 120 mg/L at 1–2 hours post-ingestion. Creatine has a fairly short elimination half-life, averaging just less than 3 hours, so to maintain an elevated plasma level it would be necessary to take small oral doses every 3–6 hours throughout the day."
does this mean that the water-holding potential should go down after 3-6 hours?