Yes. I recently had a big career change. I felt like it was the right time. I actually had applied for 3 jobs about the same time. I only got called to interview for one (which was kind of rare in itself). When I got to the interview, I was oddly not nervous and fidgety like I normally am. I was interviewed in front of a panel of like 8 people which would have normally freaked me out. I just made myself at home.
I left feeling like I had the job - which is usually a bad sign. I was right. About a year and a half later, I am very happy with my career change - for the most part.
So - be open minded, but trust your instincts.