Not only will shoes make a difference, biking shorts will as well.
Man, did I learn the hard way. Saddle rash... painful!
Found this to be extremely helpful: wear real cycling shorts and before you put them on, put a little Astroglide on the inside of each thigh - then, after spinning/cycling, get out of them and shower - wash shorts after each ride, but air dry - problem solved!
Spin on, my friend, spin on!