Melbourne Florida, or Australia? But since you said "pub", I'm going for Australia.
As for Melbourne Australia, all I can think of and know of is the "laird" Gibbs St, in Abbotsford/ Collingwood. It's also big with the bears and leather queens too, and thats not really straight. Flamers where once never welcome, but they come looking for a man, but not on mass as many also feel intimidated. It's got a pool room, beer garden and a few bars, and by law, women are not pormitted, as it's a men's only pub!
There is a pub, or what was once one in Commercial Rd, South Yarra The Exchange, , that was meant to go the same way, but the Drag Queens invaded it. Such clubs are limited as they are not shell we say "politically correct"
I think also the "Peel", has an upstairs bar that is meant to be for the guys a few nights a week, but I've never been, and it's a club, and not a pub. I hear the Greyhound in St kilda is hopping, but I'm sure it's along way from straight being in that part of town. Then you have my beer garden, yet thats a private club, and not a public pub.